GeneCopoeia CytoCt RT-qPCR System

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GeneCopoeia’s CytoCt RT-qPCR System enables users to analyse gene expression by real-time RT-qPCR directly from 10–100,000 cultured cells either in tubes or 96-well plates without any RNA purification, providing high levels of convenience, speed, throughput and sensitivity.

RNA is traditionally extracted and purified from cultured cells before gene expression quantification and profiling analysis by RT-qPCR assays. The CytoCt RT-qPCR System skips the RNA extraction step and prepares cell lysates directly from cells cultured in 96-well plates or other formats.

Gene expression analysis can be completed in about 90 min using a cell lysis buffer followed by either a two-step or a one-step RT-qPCR workflow. The system includes CytoCt Cell Lysis kits, CytoCt cDNA Synthesis kits and CytoCt One-Step RT-qPCR kits.

The product provides robust performance with system optimisation from cell lysis to RT-qPCR reactions.

For more information: https://www.genecopoeia.com/product/cytoct-rt-qpcr-system/.