Logos Biosystems LUNA for counting of live and dead cells

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Cell counting allows the behaviour and viability of cells to be monitored, providing insights into bioprocessing and cell therapy dosing. Many laboratories performing transfection, cell proliferation, viability studies or quantitative PCR use manual cell counting methods like a haemocytometer, which is still considered the gold standard despite being time-consuming, tedious and subjective.

Cells are notoriously challenging to count, even by an experienced analyst. They can exhibit broad size distributions (mesenchymal stem cells), contain debris (PBMCs), be irregularly shaped (primary hepatocytes), be clustered (MCF-7 breast cancer cells) or contain budding yeast cells. Automated cell counters are designed to offer results in a fraction of the time.

Not all cell counters are created equal, as there are many different instruments. Three systems in the LUNA automated cell counter family are available for demonstration: the LUNA Brightfield (7 s to results), LUNA FL (GFP transfection) and LUNA FX-7.

The LUNA-FX7 is the latest member of the family and is claimed to provide unmatched cell counting accuracy, dual fluorescent and brightfield detection, an advanced de-clustering algorithm, precision autofocus and 21 CFR PART 11 compliance. It has built-in quality control features and precise validation slides for monitoring QC and bioprocesses.

Key benefits include: larger counting volumes, up to 5.1 µL for high accuracy (1% CV); high throughput using either 8- or 3- channel slides; the convenience of disposable and reusable slides; bioprocess package, CountWire and 21 CFR Part 11 compliance; and intuitive operation.

For more information: https://www.atascientific.com.au/products/luna-fx7-automated-cell-counter/.