New England Biolabs NEBNext UltraShear FFPE DNA Library Prep Kit

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New England Biolabs has released the NEBNext UltraShear FFPE DNA Library Prep Kit (NEB #E6655), a solution for working with challenging formalin-fixed paraffin-embedded (FFPE) samples in molecular labs. FFPE DNA has long posed difficulties due to its low input amounts, non-uniform ends and DNA damage caused by fixation and embedding. However, the NEBNext UltraShear FFPE DNA Library Prep Kit offers a streamlined, user-friendly workflow that minimises hands-on time and addresses these challenges.

The kit incorporates the NEBNext FFPE DNA Repair Mix v2, designed to selectively target damaged DNA bases and enhance data accuracy while improving library conversion rates. It effectively rectifies issues like nicks, gaps and overhangs, resulting in good library quality.

Additionally, the NEBNext UltraShear FFPE DNA Library Prep Kit prevents over-fragmentation and retains intact DNA by filling in single-stranded overhangs that are free from damage, enabling optimal sequencing coverage. Importantly, the kit preserves true mutations while removing damaged bases, preventing false-positive results in mutation analysis.

The kit offers a sample-quality-agnostic workflow with a broad input range, making it suitable for high-throughput settings such as clinical labs. Enzymatic fragmentation is automation-friendly and less dependent on sample quality, providing consistent results.

With the NEBNext UltraShear FFPE DNA Library Prep Kit, researchers can unlock valuable genetic insights from FFPE samples — regardless of their quality or quantity — putting an end to compromised library preparation and sequencing results and resulting in efficient and consistent FFPE DNA analysis.

Learn more at www.neb.com/LLS-FFPE.