Beckman Coulter protein characterisation application note
The application note ‘Assay Of IgG Purity And Heterogeneity Using High-Resolution Sodium Dodecyl Sulfate Capillary Gel Electrophoresis’ highlights the development of both a new polymer formulation and standardised methodology to assess the purity and heterogeneity of IgG and its isoforms.
The methodology provides increased resolution of the IgG isoforms from typical developmental impurities like non-glycosylated heavy chain and low-molecular-weight impurities associated with the IgG light chain.
The assay includes an artificially engineered IgG control with a fixed percentage of non-glycosylated heavy chain to provide assay suitability determination prior to the analysis of unknowns. All aspects of the methodology from preparing samples to automating data analysis are discussed.
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