Enzo FLUOFORTE Calcium Assay Kits

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Measurement of transient calcium mobilisation from intracellular stores in response to the activation of G protein-coupled receptors (GPCRs) is considered a standard approach to the pharmacological characterisation of receptors and compounds, frequently implemented in primary screening and lead development programs.

Enzo’s FLUOFORTE Calcium Assay Kits detect mobilisation of intracellular calcium utilising a fluorogenic calcium-binding dye optimised for good cell permeability and retention. Developed for use with conventional dual-dispensing microplate readers, the kits provide EC50 values comparable to Fluo-4 and Calcium 4.

FLUOFORTE dyes are claimed to offer two-fold brighter fluorescence vs Fluo-4, enabling the detection of weak signal compound responses. The sensitive dye provides a large assay window, allowing for the detection of even weak signal compound responses. Optional red dye enables multiplexing with green fluorophores.

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