GeneCopoeia miTarget miRNA 3′ UTR target clones

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miTarget miRNA 3′ UTR target clones from GeneCopoeia are designed for mammalian miRNA target identification and functional validation of predicted targets. Target clones are also used to study the regulatory effect of miRNA on target gene(s).

3′ UTR sequences were obtained from public domain gene sequence databases and inserted downstream of the secreted Gaussia luciferase (GLuc) reporter gene in its respective vector system, driven by SV40 promoter for expression in mammalian cells. A chimeric mRNA is transcribed consisting of the GLuc and a 3′ UTR target sequence. Thus, the study of mRNA-miRNA target interaction can be easily performed with a live cell assay for GLuc using only 10 μL of the cell culture medium.

Also available from GeneCopoeia is the dual-reporter system — secreted Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) — enabling live cell assays and transfection normalisation for accurate across-sample comparison.

For more information: http://www.genecopoeia.com/product/mirna-targets/.