LifeSensors UbiQuant Quantitative Ubiquitin ELISA
The UbiQuant ELISA from LifeSensors is intended for the accurate determination of total ubiquitin - including free ubiquitin, monoubiquitin and polyubiquitin - in biological samples, including cell lysates, tissue homogenates and plasma.
Total ubiquitin is captured in the wells of a pre-coated microtitre plate using a proprietary ubiquitin binding reagent. Unbound protein is removed by washing and then an anti-ubiquitin monoclonal antibody (VU-2) is added. After removing the unbound VU-2, the bound antibody is measured using an enzyme-linked, anti-mouse antibody.
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