Lonza ViaLight Plus cell proliferation/cytotoxicity bioassay assay kit

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The ViaLight Plus cell proliferation and cytotoxicity bioassay assay kit uses bioluminescent detection of cellular ATP as a measure of viability, claimed to be the most accurate, effective and direct way of determining the number of living cells in culture.

Dynamic range of 5 decades and good performance with either adherent or suspension cultures allows users to design an experiment for their needs.

The two-step, non-radioactive assay is scalable for high-throughput applications in both 96- and 384-well formats. Users simply add two reagents directly to the culture well and read (no cell washing or medium removal required).

Results from a 96-well plate can be processed and analysed in as little as 10 minutes on a variety of luminometers or scintillation counters.

Its features include: can detect as few as 10 cells allowing for lower seeding densities and more assays; stable bioluminescent signal (half-life >5 hours) facilitates manual or batch processing; unlike MTT or Alamar Blue assays, the bioassays are not prone to interference from culture condition changes.