The MycoAlert mycoplasma detection kit includes reagents and an instruction manual for the detection of mycoplasma contaminations in cell cultures.
MycoAlert is claimed to be simple, fast to perform (<20 minutes) and to give reliable results, even with very low levels of mycoplasma contamination.
The assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid screening procedure giving sensitive detection of mycoplasma contamination in a test sample.
Viable mycoplasma are lysed and the freed enzymes react with the MycoAlert substrate catalysing the conversion of ADP to ATP. By measuring the level of ATP in a sample both before and after the addition of the substrate, a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
The kits are supplied with a pre-packed manual with simple protocols and background information to ensure successful results.
Phone: 1300 657508
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