MycoAlert mycoplasma detection kit

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The MycoAlert mycoplasma detection kit includes reagents and an instruction manual for the detection of mycoplasma contaminations in cell cultures.

MycoAlert is claimed to be simple, fast to perform (<20 minutes) and to give reliable results, even with very low levels of mycoplasma contamination.

The assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid screening procedure giving sensitive detection of mycoplasma contamination in a test sample.

Viable mycoplasma are lysed and the freed enzymes react with the MycoAlert substrate catalysing the conversion of ADP to ATP. By measuring the level of ATP in a sample both before and after the addition of the substrate, a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

The kits are supplied with a pre-packed manual with simple protocols and background information to ensure successful results.