MycoAlert mycoplasma detection kit

Monday, 21 June, 2010 | Supplied by: Lonza Australia Pty Ltd

The MycoAlert mycoplasma detection kit includes reagents and an instruction manual for the detection of mycoplasma contaminations in cell cultures.

MycoAlert is claimed to be simple, fast to perform (<20 minutes) and to give reliable results, even with very low levels of mycoplasma contamination.

The assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid screening procedure giving sensitive detection of mycoplasma contamination in a test sample.

Viable mycoplasma are lysed and the freed enzymes react with the MycoAlert substrate catalysing the conversion of ADP to ATP. By measuring the level of ATP in a sample both before and after the addition of the substrate, a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

The kits are supplied with a pre-packed manual with simple protocols and background information to ensure successful results.

Phone: 03 9550 0883
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