Abcam knockout cell lines and lysates

CRISPR-Cas9 knockout cell lines from Abcam provide scientists with off-the-shelf, single-gene knockouts so the user can confidently interrogate the relationship between genotype and phenotype without having to establish their own knockout cell line. The company provides a large and expanding range of knockouts in immortalised mammalian cell lines, such as HeLa and HEK293T. All knockout cell lines are Sanger sequenced and many have additional western blot data to confirm knockout at the proteomic level.

Additionally, users can access over 2700 diploid knockout cell lysates, generated from commonly used cancer cell lines. They can select from a large library of immortalised diploid knockout cell lines including HeLa, HEK293T, A549, HCT116, Hep G2 and MCF7, derived from single-cell clones. Each knockout cell line is individually cloned and validated by Sanger sequencing. The knockout cell lysates are provided lyophilised with the parental wild-type lysate to allow the biological impact of the knockout to be assessed within a consistent cellular background.