Abcam knockout cell lines, lysates and pellets

Abcam’s knockout (KO) catalogue range and custom service makes it easy for scientists to get the gene-edited cell lines they need. The expertly engineered CRISPR-Cas9 KO cell lines allow users to confidently interrogate the relationship between genotype and phenotype without having to establish their gene-edited cell line.

The company’s KO cell lines have over 400 gene targets available, with multiple parental cell lines, supplied frozen as 1x 10⁶ cells/vial in 1 mL. They are suitable for cell-based assays, with a wild type cell line available at no cost.

The company’s KO cell lysates have over 2600 gene targets available, with parental cell lines including HeLa, HEK293T, A549, HCT116, Hep G2 and MCF7 derived from single-cell clones. Supplied lyophilised as 1x 100 µg, they are suitable for WB with parental wild-type lysates available.

The company’s KO cell pellets provide access to native proteins, without the need to culture cells. Prepared from single-gene KO cell lines and snap-frozen, they are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. They can be used for a variety of applications, including PCR, gene expression profiling and DNA library preparation.