AdipoGen Life Sciences BioStab PCR Optimizer

PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5′-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments.

AdipoGen Life Sciences’ BioStab PCR Optimizer solves this problem and is said to outperform betaine; its mode of action comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The product is designed as a PCR additive that improves the yield and specificity of the PCR reaction and allows amplification of difficult targets (eg, sequences with >70% GC pairs).

The product enables the use of a wide range of annealing temperatures, thereby eliminating the need for an optimisation of amplification conditions. It is provided as a 5x concentrated solution. The working concentration in the PCR mixture is 1x. For targets with >75% GC content, a 2x solution is recommended.