AdipoGen Life Sciences BioStab PCR Optimizer
PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5′-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments.
AdipoGen Life Sciences’ BioStab PCR Optimizer solves this problem and is said to outperform betaine; its mode of action comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The product is designed as a PCR additive that improves the yield and specificity of the PCR reaction and allows amplification of difficult targets (eg, sequences with >70% GC pairs).
The product enables the use of a wide range of annealing temperatures, thereby eliminating the need for an optimisation of amplification conditions. It is provided as a 5x concentrated solution. The working concentration in the PCR mixture is 1x. For targets with >75% GC content, a 2x solution is recommended.
Phone: 02 9698 2022
ALA Scientific Instruments RuFus cell unroofing system for plasma membrane isolation
ALA Scientific Instruments offers RuFus, a complete and user-friendly system for performing cell...
Lonza plateable mouse hepatocytes
Lonza provides plateable mouse hepatocytes from the four most common research mouse strains,...
PEPperPRINT cLIFT peptide microarray platform
At the core of the technology is a laser-based transfer process that is designed to deposit amino...