ALA Scientific Instruments RuFus cell unroofing system for plasma membrane isolation

ALA Scientific Instruments offers RuFus, a complete and user-friendly system for performing cell unroofing with reproducibility. Designed to streamline and simplify this advanced technique, the system integrates all the components needed to unroof and fix cells for electron microscopy workflows.

Cell unroofing is a powerful and dynamic technique used to prepare samples for enhanced cryo-microscopy. It enables researchers to visualise the ultrastructure of the cell cytoplasm by exposing the interior of cells through the controlled removal of the plasma membrane. This method involves the precise application of enzymes to the upper surface of cultured cells, allowing for high-resolution imaging of internal cellular structures. Cell unroofing is suitable for studies requiring direct access to the cytoplasmic face of membranes or organelles with minimal sample disruption.

Each purchase of the ALA RuFus cell unroofing system comes with the essential accessories needed. This includes a lab stand, X-Y positioner, Petri dish holder, stainless steel needle cannula, pressurised 60 mL reservoir, ALA Valve-1 controller, three-way pinch valve manifold, and all necessary tubing and connectors.