Aviva’s Immunohistochemistry Kit is intended to provide a simple and convenient detection system for immunohistochemical staining. As such, the kit can be used for the identification of specific constituents in tissue sections or immobilised cells. To detect the reaction site, the antibody complex is labelled with an enzyme (peroxidase) that can be reacted with a suitable substrate (DAB) to give a coloured product.
The kit also provides for the counter staining of nuclei to reveal tissue architecture. The stain and substrate colour fade slowly, if at all, allowing the slides to be stored for future use.
The contents of the kit are formulated for maximum ease of use and are colour-coded to eliminate confusion. A sample protocol and recommended conditions for use are provided.
Formaldehyde fixation is suggested as the routine initial method of choice for tissue and cell fixation. The provided protocol is a guideline. Any procedure can be altered according to specific experimental requirements.
The use of both highly purified and adsorbed antibody conjugates and matched reagents reduces any lot-to-lot variation. The kit can process 100–200 immunostains on standard microscope slides.
Phone: 02 9698 2022
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