ELISA (enzyme-linked immunosorbent assay) is a commonly used plate-based assay technique first described in the 1970s. ELISAs are used to measure antibodies, antigens and proteins that are found in biological samples, such as serum, cell lysates and other fluids.
While in-house development of ELISA assays is possible, most researchers buy validated ELISA kits to simplify their workflow. A common ELISA kit format is the sandwich ELISA, where the assay uses a capture antibody to bind the target antigen found in a sample and a detection antibody that is labelled with an enzyme that converts the assay substrate, such as TMB, into a coloured product that can be measured using a plate reader.
Aviva Systems Biology offers over 18,000 ELISA kits for nearly 7000 antigen targets, hormones and molecules across a broad range of species such as human, mouse, rat, bovine, pig, dog and more. Research customers can find ELISA kits for both novel and common targets, using the company’s online site search to find the most suitable ELISA kit for their target.
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