Beckman Coulter Life Sciences SPRIworks HT automated sample preparation system

Sunday, 25 March, 2012 | Supplied by: Beckman Coulter Australia

SPRIworks HT, from Beckman Coulter Life Sciences, is a high-throughput solution for fragment library preparation on Illumina next-generation sequencers. The product allows researchers to process libraries with greater speed and sample reproducibility and decrease processing costs. The system uses built-in SPRI (Solid Phase Reversible Immobilisation)-based per-well size selection.

User interaction is seamless and requires as little as 15 min of hands-on time. Up to 96 samples can be prepared in as little as 3 h without size selection, or 6 h with size selection. The simplicity of the system enables users to seamlessly perform automated sample preparation protocols upon their first interaction with the system.

The product offers a reagent kit for library preparation that includes sizing solution and PCR reagents. Protocols are automated via a suite of methods built on the Beckman Coulter Biomek FXP Liquid Handling Workstation. Per-well size selection provides users with the flexibility to process samples for multiple applications in a single run.

For added efficiency, methods are also included for downstream processes such as PCR setup, PCR cleanup, qPCR setup, quantitation, normalisation and sample pooling. The overall system increases reproducibility, decreases processing costs and eliminates most manual preparation steps in next generation sequencing sample prep.

The SPRIworks HT system complements existing SPRIworks platforms, SPRI paramagnetic bead-based chemistry and Biomek liquid handling platforms, which are used to support genomic researchers in their work to solve biological problems through sequencing.

Phone: 1800 060 878
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