Beckman Coulter MoFlo Astrios cell sorter

Thursday, 03 February, 2011 | Supplied by: Beckman Coulter Australia

The MoFlo Astrios next-generation cell sorter leverages the stable fluidic design of the original MoFlo and the electronic processing of the MoFlo XDP, while expanding sorting capabilities with an array of laser options. A range of software components further enhances instrument functionality.

Complex, multicolour sorting is enabled by seven pinholes that spatially separate each of the seven lasers. Auto start-up allows the researcher to define when lasers and fluidics turn on, saving time. The multifibre beam shaping optic creates flat-top beam profiles, providing short alignment times and high-level optic stability. Two stages control the alignment for all seven lasers to simplify sorter preparation.

Beckman Coulter’s IntelliSort II is a bead-less drop delay determination and monitoring system. Camera images and proprietary algorithms determine optimal drop delay without fluorescent beads being incorporated in the sample line. A quick-release nozzle design enables easy removal or swapping of the nozzle body and tip through the use of a single lever.

The auto-quality control allows system performance to be tracked and trended across 32 available parameters, while the updated Summit Software permits six-way sorting into tubes and sorting into plates having as many as 1536 wells. Index-sorting logic tracks each plate-sorted cell back to its histogram or scatter plot. A touch-screen control panel facilitates daily set-up with operations designed around core laboratory workflow.

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