Bio-Rad IncRNA Workflow

Bio-Rad Laboratories has announced the IncRNA Workflow, a RT-qPCR workflow that is optimised for highly sensitive and specific quantification of long noncoding RNAs (lncRNAs) for gene expression analysis. The workflow provides a streamlined alternative to RNA-Seq for lncRNA discovery and validation.

The product solves the most pressing challenges in lncRNA discovery and validation with its workflow that includes PrimePCR lncRNA Assays, Predesigned PrimePCR lncRNA Arrays, Custom PrimePCR lncRNA Arrays, and the novel iScript Explore One-Step RT and PreAmp Kit. The workflow offers all the tools necessary to go from experimental sample to lncRNA data analysis.

Researchers can use iScript Explore One-Step RT and PreAmp Kit to preamplify low-abundance lncRNAs, a step that expands the number of targets that can be assayed from limited sample material. The kit allows researchers to perform genomic DNA digestion, reverse transcription and preamplification. Even difficult targets can become readily detectable with the included reaction booster technology.

Bio-Rad’s 20 predesigned PrimePCR lncRNA Arrays cover the most active fields for lncRNA research, including cancer, immunology, stem cell biology and more. The predesigned arrays are curated to include a combination of frequently cited lncRNAs and novel lncRNAs identified through bioinformatic analysis to aid in target discovery. The plates can be paired with the company’s Preblended lncRNA PreAmp Assay Pools, which contain all assays necessary to preamplify targets on the corresponding predesigned array, thereby reducing set-up time.

The workflow also offers solutions for researchers who have already identified lncRNAs they wish to study and who want to validate or expand their discovery work with a larger group of samples. Custom PrimePCR lncRNA Arrays can be designed to include anywhere from 10 to 384 lncRNA and mRNA targets per array. When desired, researchers can also include self-designed individual assays in custom arrays.

The workflow is fully compatible with Bio-Rad’s CFX Real-Time PCR Detection Systems with CFX Maestro Analysis Software, SsoAdvanced Supermixes and plastic consumables.