Bio-Rad Laboratories Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody Assays

Bio-Rad Laboratories has announced the Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody Assays, for research use only (RUO). The assays allow scientists to measure neutralising antibodies quickly and efficiently against wild-type and significant variants of SARS-CoV-2 virus.

Scientists developing vaccines and therapeutics can determine the efficacy of their product from development through all the clinical phases. Epidemiologists can perform seroprevalence studies to better understand the longevity of the neutralising antibody response from infection or vaccination. Researchers can increase their throughput when compared to using traditional cell-based assays and ELISA tests, the company claims.

The assays are offered in an 11-plex, complete, all-in-one kit format that consists of the wild-type RBD and S1 and 9 variant antigens, as well as customisable singleplex assays, 2-plex Delta variant coupled beads and a custom Assay Developer Kit to create assays to any new SARS-CoV-2 variant — such as Omicron — for maximum flexibility.

The Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody 11-Plex Panel is a ready-to-use, 96-well kit containing premixed magnetic capture beads, a detection ACE2 receptor, a standard and positive control, and buffers that measure levels of neutralising antibodies and percentage inhibition of the ACE2 receptor binding the viral antigens coupled on the beads. The assay requires only 15 µL of sample when running duplicates.

The singleplex variant coupled beads, and 2-Plex Delta RBD and Spike Trimer coupled beads with positive control, enable measurement of neutralising antibodies specific to viral variants of interest. They should improve laboratory efficiency by generating key actionable data in a single experiment.

The assays are compatible with RUO Bio-Rad platforms, including the Bio-Plex 200 and Bio-Plex 3D Multiplex Immunoassay Systems, as well as all other RUO xMAP platforms.