Bio Rad SingleShot cell lysis RT-qPCR kits

Bio-Rad Laboratories has announced the launch of a rapid cell lysis kit that allows researchers to obtain reverse transcription quantitative PCR (RT-qPCR) data directly from cultured cells without the need for a separate RNA purification step. The SingleShot family of cell lysis RT-qPCR kits provide high-quality gene expression results in less than 2 h.

Available column isolation methods for purifying RNA are said to be time consuming and laborious, while other methods that enable RT-qPCR directly from cell lysates can damage the RNA and result in poor genomic DNA clearance. The kits eliminate such challenges and offer good reproducibility and accuracy of gene expression results. Minimal set-up and pipetting steps create an automation-friendly workflow and, unlike other similar methods, the kits do not require an additional pipetting step to stop the cell lysis reaction.

The kits are suited for high-throughput laboratories with large-volume workloads and for researchers who are faced with a limited number of cells and require high accuracy in each analysis. They include an RNA control template and qPCR assay to help researchers determine optimal cell number and lysate inputs for their RT-qPCR reactions.

The kits are validated for use with a wide variety of adherent and suspension cell lines and are offered in multiple formats, including one-step RT-qPCR and two-step RT-qPCR kits that are compatible with either SYBR Green or probe-based assays. They are also available as stand-alone cell lysis kits.