BioStatus DRAQ7 far-red fluorescent DNA dye

DRAQ7 is a non-toxic, far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilised cells. The dye does not overlap with PE and homologues, making it a suitable replacement for common dyes such as propidium iodide and 7-AAD in cytotoxicity and cell viability assays.

The product is a highly photostable, pure synthetic compound which can be used in most cell types, including mammalian, bacterial, parasitic, and plant cells and tissues. The versatile dye can be used in a variety of applications including immunofluorescence and immunohistochemistry, high content screening (eg, flow cytometry) and cell-based assays.

The dye is easy to use and does not require washing or RNase. It does not enter intact live cells and is non-toxic in long-term culture. Its far-red fluorescence enables counterstaining or dual staining with other common labels, such as GFP and FITC. Furthermore, the dye provides differential staining of the cytoplasm to permit dual compartment segmentation for translocation tracking and cell morphometrics.

The product’s spectral profile is Exλ_max 599/644 nm, Emλ_max 678 nm/694 nm intercalated with dsDNA.