Cayman Chemical Cellular Fatty Acid (C16) Uptake Assay Kit

The transport of fatty acids across the cell membrane is mediated by various membrane-associated fatty acid-binding proteins that both facilitate and regulate cellular fatty acid uptake. Dysregulation of fatty acid transport, including increased transport of palmitic acid, is correlated with the development of chronic kidney disease (CKD), non-alcoholic steatosis (NASH), non-alcoholic fatty liver disease (NAFLD), obesity and diabetes.

Cayman Chemical’s Cellular Fatty Acid (C16) Uptake Assay Kit is a convenient tool for studying cellular uptake of the fatty acid palmitate. The kit employs BODIPY-palmitate, a fluorescently tagged palmitic acid, as a probe for monitoring the uptake of fatty acid into live cells. It can be used to screen inhibitors of fatty acid uptake and includes lipofermata, a potent inhibitor of FATP2, as an inhibitor control.

Each kit includes validated protocols for kinetic uptake, endpoint uptake and fluorescence imaging, and provides enough BODIPY-palmitate for 100 tests in a 96-well format.