Cayman Chemical Cyclic AMP TR-FRET Kit

Cyclic adenosine monophosphate (cAMP) is an intracellular signalling molecule that is a key mediator in a variety of pathways, including glycogen metabolism, gene regulation and olfactory sensory transduction.

Cayman’s Cyclic AMP TR-FRET Kit is designed to allow the user to detect and quantitatively measure the amount of cAMP present in cell-based assay systems. A europium (Eu) labelled monoclonal anti-cAMP antibody (donor) is paired with a fluorescence labelled cAMP analogue (acceptor). In the absence of any endogenous cAMP, it will produce a time-resolved energy transfer signal at 665 nm when excited at 340 nm. If cAMP accumulates in the well due to the activation of cellular adenylyl cyclase, this cAMP inhibits the formation of the labelled donor/acceptor complex, decreasing the amount of energy transfer signal produced, with the overall drop in signal being inversely proportional to the amount of cAMP accumulated.

The reagents supplied in the kit run up to 1000 assays in 384-well plates or 2000 assays in 1536-well plates.