Cayman Chemical STAT3 TR-FRET kits

Cayman Chemical’s STAT3 TR-FRET kits use a homogeneous TR-FRET assay method for rapid measurement of phosphorylated and/or total STAT3 protein levels in cells.

STAT3 is a ubiquitously expressed transcription factor that has roles in a variety of cellular functions including proliferation, apoptosis and differentiation, as well as innate and adaptive immunity. Phosphorylation of cytosolic STAT3 at tyrosine 705 (Tyr⁷⁰⁵) in response to stimulation with cytokines or growth factors induces STAT3 dimerisation and nuclear translocation, leading to STAT3-dependent gene transcription.

Cayman offers three STAT3 TR-FRET kits: the STAT3 (Phospho-Tyr⁷⁰⁵) TR-FRET Kit measures the levels of STAT3 phosphorylated at Tyr⁷⁰⁵ (phospho-Tyr⁷⁰⁵); the STAT3 (Total) TR-FRET Kit measures total STAT3 levels; and the STAT3 (Total) and STAT3 (Phospho-Tyr⁷⁰⁵) TR-FRET Kit measures the levels of both. The streamlined ‘add-incubate-measure’ assay protocol is designed to decrease hands-on time and provide a powerful alternative to more error-prone and time-consuming techniques such as Western blot and ELISA.

The STAT3 TR-FRET kits exhibit low background fluorescence levels, a stable assay readout and high signal-to-background ratios. Each assay kit contains the appropriate TR-FRET antibody pair(s), as well as buffers and a positive control lysate. The robust assays are suitable for screening a large number of samples and are compatible with both 96- and 384-well formats.