Cayman Chemical Testosterone ELISA Kit

Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs, including muscle, liver and brain, directing the development of the male phenotype during embryogenesis and at puberty.

Testosterone is synthesised from 17α-hydroxy progesterone by the enzymes 17,20-lyase and 17β-hydroxysteroid dehydrogenase in the gonads of both males and females. In many target cells it is reduced to 5α-dihydro testosterone, which mediates many of the biological actions of testosterone. It is then further metabolised to 17β-oestradiol by aromatase. Serum levels of testosterone range from 0.5 ng/mL in women to approximately 6–10 ng/mL in men, declining with age.

Cayman Chemical’s Testosterone ELISA Kit is a competitive assay that can be used for the quantification of testosterone in serum, plasma, tissue culture supernatants and other sample matrices. The assay has a range from 3.9–500 pg/mL and a sensitivity (80% B/B₀) of approximately 6 pg/mL.