DiLYX OptiSol protein solubility screening kits

OptiSol protein solubility kits from DiLYX are plate-based arrays designed for screening protein stability and buffer optimisation. The kits can be used with proteins from 5 to 300 kDa, identifying formulations which prevent aggregation or are able to de-aggregate specific proteins.

Proteins often aggregate when exposed to elevated temperature, intense light, freeze-thaw cycles and extended storage. The kits contain a systematically varied array of buffers (from pH 3 to pH 10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that elucidate the conditions which prevent aggregation, or are able to de-aggregate proteins. A total of 90 different formulations with solubility enhancers can be tested in one single, label-free experiment.

The kits follow a simple mix-and-spin protocol that can be completed in under 2 h and require low sample input (less than 10 µL for each protein solubility data point). The kit is compatible with many functional assays, SDS-PAGE, Western blot, mass spectrometry and fluorescence-based protein.

There are two kits available, depending on the user’s target protein: OptiSol 30, for proteins 5 to 50 kDa; and OptiSol 200, for proteins 50 to 300 kDa.