DNA fragment cloning

MultiSite Gateway Pro Technology enables users to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway Expression vector.

Using specifically designed att sites for recombinational cloning, users can clone two, three or four DNA fragments into any Gateway Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The MultiSite Gateway Pro Technology allows users to: clone multiple DNA fragments into one vector without using restriction enzymes or ligases; take full advantage of the wide selection of Gateway Destination vectors; and replace multiple plasmid transfections with a single vector carrying all DNA elements of interest.

MultiSite Gateway Pro Kits are available for cloning two, three or four DNA fragments and for flexible and customised configuration of DNA fragments into a Gateway Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.