DNA/RNA purification system

Tuesday, 07 April, 2009 | Supplied by: Phenomenex Australia

Phenomenex’s Clarity QSP system for synthetic RNA and DNA purification uses trityl-on purification to deliver concentrated, full-length oligonucleotide sequences.

The system is offered in three cartridge formats and in 96-well plates. The cartridge formats are suitable for applications that require increased loading capacities, purifying crude oligo samples in under 8 mins using vacuum or positive pressure systems. The 96-well plate format can be easily automated for high-throughput parallel purification and eliminates the tube-in-plate combination of other approaches.

The system overcomes the pitfalls of first-generation trityl-on purification. Its unique buffer works synergistically with ammonia-based deprotecting solutions to selectively retain the full-length trityl-on DNA or RNA sequence. The method eliminates subsequent wash steps and does not require toxic ion pairing reagents.

DNA is delivered at 90 to 95% purity levels, with reliable recovery rated of more than 80%. RNA purification with Clarity QSP boats 89 to 93% purity levels with reproducible recovery values of more than 75%.

The cartridges and plates are part of the Clarity BioSolutions portfolio of products for synthetic oligonucleotide purification. From low purity/low loading to high purity/high loading tools, the manufacturer offers a range of solutions along with hands-on technical and applications support.

Online: www.phenomenex.com
Phone: 02 9428 6444
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