Enzo Life Sciences calcineurin cellular activity assay kit

Calcineurin is a eukaryotic Ca²⁺ and calmodulin-dependent serine/threonine protein phosphatase. It activates the NFAT transcription factor, stimulating the T-cell response through IL-2 expression. Calcineurin is involved in immune cell signalling and is a target for inhibitors, such as everolimus, which are used in heart transplantation to prevent rejection.

Enzo Life Sciences provides a complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin phosphatase activity in tissue/cellular extracts. Human recombinant calcineurin is included as a positive control.

The RII phosphopeptide substrate supplied with the kit is claimed to be the most efficient and outstanding peptide substrate known for calcineurin. The detection of free-phosphate released is based on the Malachite green assay and provides a sensitive and safe non-radioactive assay with convenient one-step detection.