Enzo Life Sciences GFP-CERTIFIED Apoptosis/Necrosis Detection Kit

The transition from apoptosis to necrosis is a loosely defined continuum that requires an appropriate recognition of the various stages of the process. The GFP-CERTIFIED Apoptosis/Necrosis Detection System from Enzo Life Sciences was specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins.

The kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labelled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein or coumarin. Kit components include an Annexin V-EnzoGold (enhanced Cyanine-3) conjugate that enables detection of apoptosis distinct from fluorescein or GFP.

The Necrosis Detection Reagent (Red), similar to the red-emitting dye 7-AAD, facilitates late apoptosis and necrosis detection. The detection reagents can be used separately or in combination for either single or multiplexed applications respectively.

The kit also provides quick results via flow cytometry and fluorescence/confocal microscopy. It is particularly useful to researchers aiming to effectively distinguish between apoptosis/necrosis in their chosen sample type.

Featuring true multiplexing capabilities with GFP and other green fluorescent probes, the product readily distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells. It is optimised for both fluorescence microscopy and flow cytometry applications and is suitable for death pathway analysis and drug/toxin studies.