EpiGentek EpiQuik CRISPR/Cas9 Assay ELISA Kit (Colorimetric)

CRISPR/Cas9 allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided Cas9 nuclease and appropriate guide RNAs (gRNA) into a cell. It is important to monitor the level of Cas9 editing protein or track the Cas9 editing protein in transfected cells, as it will tell transfection efficiency and optimise the editing process in the total cell population.

The EpiQuik CRISPR/Cas9 Assay ELISA Kit (Colorimetric), from EpiGentek, is suitable for specifically quantifying CRISPR-associated protein 9 (Cas9 and dCas9: S. pyogenes) in purified Cas9 nuclease and whole cell extracts isolated from tissues and cultured cells of various species. With a quick and efficient 3 h procedure, the kit provides an innovative colorimetric assay to quantitatively measure Cas9 protein.

The product is suitable for screening Cas9-transfected clones, with a detection limit as low as 0.1 ng/well. Offering simple and consistent assay conditions, the flexible strip microplate format can be used for manual or high-throughput analysis.