EpigenTek MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of optimised buffers and reagents to colorimetrically quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, one-step, ELISA-like reaction. With its reduced number of required steps, the entire procedure needs only 2 h.

The kit composition has been designed to allow the assay to have a greater ‘signal window’ with reduced variation between replicates. It offers inherently low background noise, thereby eliminating the need for DNA denaturation and plate blocking steps. The strip-well microplate format makes the assay available for manual or high-throughput analysis.

The detection limit can be as low as 0.05% methylated DNA from 100 ng of input DNA. There is also high specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.

Positive and negative controls are included and allow for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA. The optimised positive controls can be fractionalised in percentage scale, allowing the assay to be comparable with HPLC-MS analysis.