EpigenTek MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)

5-hydroxymethylcytosine (5-hmC) is a modified form of cytosine, recently discovered in animal tissues. The function of 5-hmC in epigenetics may be different from its forerunner 5-methylcytosine (5-mC) and currently remains a mystery. It is believed, though, that 5-hmC plays an important role in switching genes on and off.

EpigenTek’s MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) is a complete set of optimised buffers and reagents to fluorometrically quantify global DNA hydroxymethylation by specifically measuring levels of 5-hmC without cross-reactivity to methylcytosine and unmethylated cytosine.

The kit includes a fluorometric assay with easy-to-follow steps for convenience and speed; the entire procedure can be completed within 3 h and 20 min. It also has high sensitivity, with a detection limit as low as 10 pg for hydroxymethylated DNA and accepting as low as 20 ng of input genomic DNA.

Universal positive and negative controls are included, which are suitable for quantifying hydroxymethylated DNA from any species. A strip-well microplate format makes the assay flexible for either manual or high-throughput analysis. Assay conditions are simple and consistent.