Epigentek MethylFlash Methylated DNA Quantification Kit (Colorimetric)
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) from Epigentek quantifies global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like, microplate-based strip-well format. It is suitable for manual or high-throughput analysis.
The colorimetric assay has easy-to-follow steps for convenience and speed, with 4 h duration. It has low background signals, eliminating the need for plate blocking. The product’s high sensitivity means it can detect as low as 0.2 ng of methylated DNA from just 50 ng of genomic DNA.
Reagents have high specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no, or negligible, cross-reactivity to hydroxymethylcytosine. Universal positive and negative controls are included, quantifying methylated DNA from any species.
Phone: 02 9698 2022
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