EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

EpiGentek’s EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) is a complete set of optimised buffers and reagents for the quantification of N6-methyladenosine (m6A or 6mA) in RNA. It is suitable for a direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria and viruses.

The kit is a colorimetric ELISA-like assay using an m6A antibody with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 h and 45 min. It has high sensitivity, of which the detection limit can be as low as 10 pg of m6A.

The binding solution allows RNA >70 nucleotides to be tightly bound to the wells, which enables quantification of m6A from both mRNA and non-coding RNA (ncRNA) such as tRNA, rRNA and snRNA. The reagents do not cross-react with unmethylated adenosine within the indicated concentration range of the sample RNA.

Universal positive and negative controls are included, which are suitable for quantifying m6A from any species.