Sigma-Aldrich has introduced a tissue PCR kit that contains reagents to rapidly extract genomic DNA from tissue or cells and amplify targets of interest by PCR. Extract-N-Amp Tissue PCR kit (Product number XNAT) offers a single-step extraction of tissue genomic DNA in less than 15 minutes.
The extraction method eliminates the need for long enzymatic digestions or homogenisation. The kit also includes a specially formulated hot start PCR ReadyMix for amplification directly from the extract. This formulation uses TaqStart antibody for specific hot start amplification, and contains the same inert red dye found in REDTaq. This red dye acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
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