Fluxion Biosciences IsoFlux System for CTC analysis

The IsoFlux System, from Fluxion, is an automated enrichment system used to isolate circulating tumour cells (CTCs) and other rare cells from biological samples prepared for downstream molecular analysis. Using microfluidic technology, the system has high CTC recovery and sample integrity, with diverse analysis options compared with conventional cell sorting technologies.

It is said to be the only system that can detect CTCs in peripheral blood at concentrations of around 1 CTC in a billion blood cells. With access to a wide range of sample types including whole blood, fractionated blood, dissociated tissue and cell cultures, the user can capture cells using single or multiple antibodies with either prevalidated or user-defined kits.

The system requires only minimal sample dilution, allowing recovery of target cells that are highly concentrated with low-volume samples and thereby producing high yield and purity of cells that are ready for molecular and cell-based analysis. The unit supports a host of downstream applications including enumeration, immunofluorescence, fluorescence in situ hybridisation, next-generation sequencing, mutation detection and gene expression.