Profinity eXact fusion-tag system’s purification resin is part of a complete expression and purification system that simplifies affinity purification and tag removal of fusion-tagged proteins, while avoiding adding time and reagent costs to the purification process. With cleavage incubation times occurring in as little as 30 mins, the system gives researchers a way to purify and cleave their fusion proteins in a single elution step, without the need for proteases.
The system is an integrated wet of products that address bottlenecks in the tag-removal process involved with the purification of affinity-tagged proteins. The result of the entire process is a single-step purification in which the user can avoid the challenge and expense of cleavage enzymes, incubation times or removal or reagents.
Key benefits of the Profinity eXact system include: purification and processing of fusion-tagged proteins in a single step; on-column cleavage in as little as 30 mins; elimination of a protease addition step; and precise cleavage at the N-terminus to generate a native protein sequence.
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