Gene expression sample preparation

Wednesday, 04 April, 2007 | Supplied by: Beckman Coulter Australia

Biomek 3000 GeXP Methods for automated gene expression sample preparation is a software plug-in that provides methods to prepare samples for the GenomeLab GeXP Genetic Analysis system on the Biomek 3000 Liquid Handling Workstation. The suite includes fully validated methods for RNA sample prep, quantitation and normalisation and reaction set-up. When used together, the Biomek 3000 workstation and these validated methods along with the GeXP Genetic Analysis system deliver a complete solution for gene expression analysis, from sample to answer.

The Biomek 3000 GeXP Methods replaces manual configurations for increased throughput, enabling the processing of 96 samples in less than four hours. The automated sample prep component incorporates the Agencourt RNAdvance Cell chemistry, based on SPRI (solid phase reversible immobilisation) paramagnetic bead-based nucleic acid purification technology.

GenomeLab GeXP is claimed to deliver more genes per reaction and more samples per run than real-time PCR, removing bottlenecks in gene expression studies for drug discovery and development research. The system's priming strategy overcomes the biases that limit standard multiplexed PCR analysis to just a few genes at a time.

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