GeneCopoeia OmicsArray protein microarrays

GeneCopoeia’s OmicsArray protein microarrays are powerful tools for many applications, including autoantibody detection, biomarker profiling and characterisation of protein–molecule interactions. Protein microarrays enable parallel detection of antibodies or other biomarkers present in patient samples for diseases including rheumatoid arthritis, muscular dystrophy, systemic lupus erythematosus and type 1 diabetes.

Each array contains high-quality purified proteins spotted onto nitrocellulose filters, which are adhered to glass slides. Proteins known to be associated with specific diseases are chosen based on a thorough review of peer-reviewed publications. In addition to predesigned arrays, arrays containing customised sets of proteins are available, as well as array profiling services and data analysis.

The protein microarrays contain up to 120 purified proteins, compared with one protein at a time for ELISA. In addition, eight spots are included for normalisation. Each slide carries 16 identical arrays, and so can be used to process up to 15 samples simultaneously as well as a negative control.

Each array can detect as little as 1 pg/mL of antibody, which is 100-fold more sensitive than ELISA. As little as 1 uL of serum or 50 uL of other bio fluids is needed for detection, and the time from sample to data can be as little as two weeks.