Illumina iSeq 100 Sequencing System

The iSeq 100 Sequencing System is a small and accessible sequencer from Illumina. Designed for simplicity, it allows labs of all sizes to sequence DNA and RNA at the push of a button. It leverages complementary metal-oxide-semiconductor (CMOS) technology and sequencing by synthesis (SBS) chemistry, enabling virtually any lab to acquire powerful next-generation sequencing (NGS) technology.

The system allows users to work across a range of applications within several areas of interest, including cancer research and microbiology, whether they are new to next-generation sequencing, sending samples out or they already have an Illumina system in the lab. It is suitable for small whole-genome sequencing (eg, bacteria, viruses, plasmids), targeted sequencing of a set of genes or gene regions, gene expression analysis or 16S metagenomics.

The product enables users to prepare libraries for a range of targeted applications, including microbial sequencing and targeted resequencing. A run on the system can be commenced in less than 5 min, with sequencing complete in 17.5 h for a 2 x 150 bp run.

Users can monitor and analyse sequencing runs using the on-instrument software and touch-screen interface. They can also interface with the system from any web browser to review real-time data and performance metrics.