Innova Biosciences Check&Go! Conjugation Test Kits

The Innova Biosciences Check&Go! Conjugation Test Kits allow scientists to confirm the success of their antibody conjugation in one easy step. The key component of the kit is a nitrocellulose membrane containing a ‘test line’ of immobilised Protein A and Protein G called a ‘half strip’.

Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The half strips also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. This simple qualitative lateral flow assay does not require any specialised equipment.

Three versions of the kits are available: Conjugate Check&Go!, to confirm the successful conjugation of a coloured label to an antibody; Biotin Check&Go!, to confirm the successful biotinylation of an antibody; and HRP Check&Go!, to confirm the successful conjugation of horseradish peroxidase (HRP) to an antibody.

The test kits are easy to use, enabling users to check the success of their conjugation in only minutes. Each test requires only small volumes to run — 20 to 40 µL of diluted conjugate. There are 30 tests per kit and all components to run the tests are provided.