Innova Biosciences Lightning-Link rapid antibody labelling system

The Lightning-Link rapid antibody labelling system from Innova Biosciences allows conjugations to be set up in seconds and the labelled antibody used within minutes.

By circumventing the desalting or dialysis steps that commonly interrupt traditional protein labelling procedures, it can be used to label small quantities of antibody with 100% recovery of the labelled antibody. Labelled antibodies are suitable for Western blotting, ELISA, immunohistochemistry, immunofluoresence and FACS analysis.

Preparing the labelled antibody is straightforward and is completed in 20 minutes by adding the antibody modifier to the purified antibody, before adding the modified antibody solution to the rapid reagent. This is then incubated at ambient temperature for 15 minutes. Finally, add the quencher; after another four minutes, the labelled antibody is ready to use.

The kit is available with the following labels: Fluorescein, Cy3, Cy5, Atto488, Atto565, Atto594 and Atto633 with more labels being added. The original antibody labelling kits are available with a range of labels that includes enzymes, fluorescent proteins, biotin/streptavidin, tandem labels and over 30 fluorescent dyes.