Integrated DNA Technologies oPools Oligo Pools

Integrated DNA Technologies (IDT) has launched oPools Oligo Pools — ready-to-use pools of high-quality DNA oligonucleotides (oligos) up to 350 nucleotides in length. The company claims to provide uniform oligo pools at yields that accelerate experiments by eliminating the need for time-consuming and bias-producing PCR amplification steps required by other commercially available oligo pools.

IDT’s manufacturing platform rapidly produces long oligos, enabling timely delivery of high-fidelity and uniform oligonucleotide pools. The pools’ low error rate of less than 1 in 2000 nucleotides, coupled with high per oligo yields, allows researchers to proceed immediately into their downstream workflows for projects including CRISPR library construction, protein screening and gene assembly. The pool deliveries are in picomoles, eliminating the need to do any pre-amplification or other pre-work before the pool can be used.

IDT employs a solid-support manufacturing platform that individually synthesises every oligo, delivering a high percentage of full-length DNA. The company’s manufacturing approach also ensures that each species of oligo is represented and at good uniformity in the resulting pool, allowing for a complete experiment downstream. 95% of sequences in the oligo pools are represented within 1.5x of the mean.