Integrated DNA Technologies rhAmpSeq targeted sequencing system

Integrated DNA Technologies (IDT) introduces its rhAmpSeq targeted sequencing system, providing researchers with easy-to-use amplicon sequencing on Illumina next-generation sequencing (NGS) platforms. The system was designed to combine the power of NGS with the simplicity and value of PCR.

The chemistry of the system enables multiplexing at high levels with good on-target rates and uniform coverage, delivering correct results and accelerating projects. It has wide-ranging applications, including human disease research, sample tracking and analysis, CRISPR genome editing analysis, and agricultural biotechnology.

The suite of rhAmpSeq targeted sequencing reagents harnesses the power of IDT’s RNase H2 PCR (rhAmp PCR) technology, following the company’s rhAmp SNP genotyping system. The technology employs a RNase H2/DNA polymerase two-enzyme system coupled with RNA–DNA hybrid primers that increase the specificity of PCR amplification while minimising primer dimer formation — a challenge for other amplicon-based chemistries and the biggest limitation of PCR multiplexing. The number of required reagents is thus reduced and the streamlined, easy workflow saves time, generating NGS-ready amplicon libraries in just two simple PCR steps.

IDT has optimised the system for consistent performance across panel sizes from tens to thousands of amplicons in a single multiplex reaction, and it can be used with either a regular or high-throughput protocol to match individual needs. In addition, the system is versatile – the technology enables researchers to design large custom panels with flexible amplicon sizes that can be tailored for various applications and sample types.