Lonza 4D-Nucleofector cell transfection platform

Thursday, 20 May, 2021 | Supplied by: Capsugel Australia Pty Ltd

Lonza’s Nucleofector technology is a non-viral cell transfection method that can be used even for hard-to-transfect cells, such as primary cells and pluripotent stem cells. Now, with an updated core unit and more intuitive software, the next-generation 4D-Nucleofector platform is designed to deliver flexibility and high performance with greater ease of use.

Electroporation is an important tool with a range of applications in disease research and drug discovery, as well as in the advancement of gene therapies, immunotherapies and stem cell generation. Nucleofector technology achieves high transfection efficiency in union with high cell viability by providing electrical pulses, cell type-specific solutions and optimised protocols to achieve an electroporation approach that targets the cell’s nucleus directly. This leads to fast, reproducible and efficient results.

The 4D-Nucleofector core unit can operate up to three functional modules, allowing for tailored experimental set-ups and facilitating scale-up from low- to high-volume transfection. The family is now joined by a fully integrated 96-well unit to suit users with mid-scale transfection requirements for up to 96 samples at once. In addition, the updated core unit features an 8″ touchscreen display, enabling users to easily set up their experiments and control all functional modules via the system’s intuitive and user-friendly software. Optimised protocols are available for more than 750 different cell types and are designed to provide robust transfection conditions, which should lead to optimal results.

Small-scale protocols can be transferred to a larger scale without the need for reoptimisation, bringing together small- and large-scale transfection applications in a single system. Users can therefore achieve high transfection efficiencies with the knowledge that their protocols can be scaled as needed.

Online: www.capsugel.com
Phone: 1300 657508
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