Lonza human cord blood CD34+ haematopoietic stem cells

Lonza has expanded its human stem cell offering to provide human cord blood CD34+ haematopoietic stem cells (CB-CD34+ HSCs) in large batch sizes, meeting a rapidly expanding market need. Lots in a range of sizes are also now available with high-resolution HLA-type information, removing the requirement for cumbersome HLA screening after lot purchase.

CB-CD34+ HSCs are useful for creating humanised mouse models, which are critical for preclinical safety testing of a range of immunotherapies. The latest offering should enable mouse model developers and researchers to increase cohort sizes for bolstered model-creating capacity, simplify HLA-matched model creation and unlock efficiencies.

Humanised mice are a powerful tool for exploring how novel cancer biotherapies interact with a human-specific immune system comprising different and interacting cell types. Across the market, there is a lack of large batch sizes of these cells, restricting the number of humanised mice that can be generated per cell lot. In addition, to maximise in vivo predictivity and accuracy of results, it is beneficial for mouse models to be carefully HLA matched to the grafted tumour tissue. Without ready-typed cells available, companies face lengthy in-house HLA screening that can add weeks to timelines, with no guarantee that an unscreened batch will contain the desired HLA type.

Now, Lonza is able to offer a consistent supply of large-batch CB-CD34+ HSCs, as well as ready-typed cell lots in various sizes. As a result, users can create large mouse model cohorts of the exact HLA-type they need, expanding testing throughput capabilities and unlocking predictive results quickly.

The company’s large cell lots are >90% purity, contain ≥2 million viable cells per lot and come complete with a certificate of analysis. HLA-typed CB-CD34+ HSC lots are screened using next-generation sequencing, providing high-resolution HLA information to minimise the risk of mismatches common with standard, low-resolution serological typing methods. Cell customers will also receive the company’s global technical support, so they can quickly overcome hurdles and achieve optimal outcomes in their mouse model creation.