Lonza RAFT 3D Cell Culture System

Thursday, 09 July, 2015 | Supplied by: Lonza Australia Pty Ltd



Lonza’s RAFT (Real Architecture For 3D Tissue) 3D Cell Culture System uses a patented absorber technology to create cell layers in high-density collagen that mimic the in vivo environment. This allows for the formation of complex models and facilitates an understanding of cellular growth, differentiation and cell-to-cell interactions.

The versatile RAFT kit is available in a number of formats and is appropriate for analysis using a wide variety of imaging, biochemical and histological techniques. In combination with Lonza’s human primary cells and media solutions, the system empowers researchers to create physiologically relevant cell culture models for use across drug discovery and research applications.

The product has been designed with simple, easy-to-follow protocols that allow researchers to set up 3D cell cultures in under 1 h. The kit includes a collagen type I solution and biocompatible absorbers, which remove the medium from cell-seeded collagen hydrogels and allow researchers to control both cell concentration and matrix density. The flexible system supports a variety of formats, including 24-well, 96-well and insert-well.

The system can be utilised with one cell type or multiple cell types in parallel. Cells can be cultured within and/or on top of the collagen scaffold. The addition of permeable membrane cell culture inserts provides other extensions to the system, allowing the generation of barrier models including air-lift models. The system is compatible with a variety of cell types and has been used to successfully generate 3D cultures in a number of research areas, including oncology, toxicology and neuroscience.

Online: www.lonza.com
Phone: 03 9550 0883
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