Merck Millipore Amnis NFкB Translocation kit

The Amnis NFкB Translocation kit allows researchers to study the nuclear translocation of NFкB - a transcription factor that plays a central role in regulating key mammalian cell processes, including proliferation, inflammation, immune and stress responses. The kit uses imaging flow cytometry to obtain statistically significant quantitative assessment of NFкB translocation, as well as visual identification of the translocation at a single-cell level.

The kit, which works with cultured cell lines and whole blood cells, conveniently contains directly conjugated anti-Human NFкB monoclonal antibody, 7-AAD dye and required buffers. Using the dedicated Nuclear Localization Wizard in the IDEAS software, NFкB translocation can be studied and quantified in an objective, statistically robust manner. The kit is designed for use with the Amnis ImageStreamX Mark II and the Amnis FlowSight imaging flow cytometers - systems which combine the quantitative power of flow cytometry with the spatial information provided by microscopy.