Merck Millipore Magna ChIP-Seq preparation kit

Saturday, 18 June, 2011 | Supplied by: Merck Pty Ltd


The Merck Millipore Magna ChIP-Seq preparation kit has been designed to simplify genome-wide profiling of DNA-protein interactions by chromatin immunoprecipitation and next-generation sequencing (ChIP-Seq).

In contrast to other products that support only part of the ChIP-Seq workflow, the Magna ChIP-Seq kit is a complete solution that provides a set of validated reagents for both chromatin immunoprecipitation and next-generation library construction. These reagents, in combination with a detailed protocol and quality-control guidelines, simplify the ChIP-Seq process to allow researchers to map interactions of histones, transcription factors and other chromatin-associated proteins on a genome-wide scale.

As successful immunoprecipitation is critical to construction of the sequencing library, the kit includes positive and negative control antibodies and a set of PCR primers that can be used as in-process controls or to verify technique. The kit has been used to construct ChIP-Seq libraries from as little as 1 ng of purified ChIP DNA, meaning that DNA segments associated with low abundance proteins can be sequenced.

Online: www.sigmaaldrich.com/AU/en
Phone: 1800 800 097
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